The two most important elements of a successful IHC assay are reliable antibodies and tissue integrity. Antibodies are widely used in biomedical research, yet there are no universally accepted methods to validate them. Antibodies can be used in a wide range of applications, but the fact that an antibody is validated for a given application does not necessarily mean it will work for another.
Ensigna Biosystems recognizes that validation of antibodies for IHC is particularly challenging. To address this, we have put in place testing methods using cell pellets with known expression levels of the protein of interest. We validate commercial antibodies or your own custom antibodies (polyclonal or monoclonal). We undertake the entire process, including the generation of controls, the screening of antibodies and the optimization of the IHC assay.
Our approach exceeds the highest standards practiced in the industry. Please contact us so we can discuss our approach in details and tailor it to your needs.